Method for measuring the activity ag coupled receptor (alpha) iog (alpha) or by the flow of Ca2 + in cells.

摘要

An in vitro method for measuring the activation of a receptor coupled to the G (alpha) i- and / or G (alpha) or cells that express at least one G-coupled receptor (alpha) q, comprising the following steps: a. simultaneous treatment of the cells with an agonist of the G (alpha) q, in a quantity such that an activity of minimum value, and with an agonist coupled to G receptor (alpha) i- and / or G (alpha) or ; and b. measuring the concentration of cytoplasmic CaZ + cells; wherein the amount of the agonist of the G (alpha) q producing activity minimum that amount at which the relationship between the result of measuring the concentration of CaZ + cytoplasmic in cells when they are treated with a certain amount of agonist of the G (alpha) q the result of measuring the concentration of CaZ + cytoplasmic in cells when they are treated with the same amount of the agonist coupled to G receptor (alpha) q simultaneously with an amount of an agonist a receptor coupled to G (alpha) i- and / or G (alpha) or which is sufficient for complete activation, it is not greater than 1: 3.