PROBE FOR CONSTRUCTING PROBE POLYMER, METHOD OF CONSTRUCTING PROBE POLYMER AND UTILIZATION THEREOF

摘要

The present invention provides a method for measuring atarget gene under isothermal conditions without using enzyme. Apair of probes each having n (n =3) base sequence regionscomplementary to each other are hybridized alternately to form adouble-stranded probe-polymer. A base pair at branched sites ofeach complementary base sequence region is designed to be a G(guanine)-C (cytosine) bond, whereby a stable double-strandedprobe-polymer is formed. One of complementary portions in oneprobe is constituted to have a base sequence complementary to a partof a target gene, whereby a target gene-probe-polymer complex isformed and the target gene is measured.