PRODUCTION OF AN ASPOROGENIC STRAIN OF BACILLUS LICHENIFORMIS AND ITS USE FOR THE PRODUCTION OF KERATINASE

摘要

A recombinant bacteria and methods of making and using the same are provided. The recombinant bacteria is a recombinant Bacillus having an inactive endogenous spoIIAC gene, with said recombinant Bacillus producing greater quantities of keratinase than a corresponding wild-type Bacillus that does not have said inactive endogenous spoIIAC gene inserted therein. The Bacillus can be Bacillus licheniformis or Bacillus subtilis. The recombinant Bacillus can be a sporulation-deficient Bacillus. A feed additive including keratinase derived from the recombinant Bacillus having an inactive endogenous spoIIAC gene and methods of inactivating an infectious prion protein are also provided.