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METHOD OF PURIFYING THREE-DIMENSIONAL CULTURE AND THE THREE-DIMENSIONAL CULTURE THUS PURIFIED

机译:纯化三维文化的方法和由此纯化的三维文化

摘要

The elution of a component to be eluted from a three-dimensional culture into a washing liquor is promoted by dipping the three-dimensional culture, which has been prepared by culturing cells to be cultured with the use of a medium containing the component to be eluted, in the washing liquor free from the component to be eluted, and subsequently or simultaneously exposing a part of the three-dimensional culture, excluding the part forming the boundary with the medium, to the washing liquor. Thus, the component to be eluted contained in the three-dimensional culture can be easily eluted into the washing liquor so that the component to be eluted, which originates in the medium, can be efficiently removed from the three-dimensional culture.
机译:通过将三维培养液浸入水中来促进从三维培养物中洗脱的组分从洗涤液中的洗脱,该三维培养是通过使用含有待洗脱组分的培养基培养待培养细胞而制备的。在没有待洗脱组分的洗涤液中,随后或同时地将三维培养物的一部分(与培养基形成边界的部分除外)暴露于洗涤液中。因此,可以容易地将三维培养物中包含的被洗脱成分洗脱到洗涤液中,从而可以有效地从三维培养物中去除源自培养基的待洗脱成分。

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