Method for the detection of reactions and metabolic changes with temperature-sensitive fluorescent material

摘要

A system, method and device for the detection of reactions between biomolecules or cells and a second compound are disclosed. The invention utilizes a fluorescent material having a fluorescence that changes with temperature. The fluorescent material is associated with a substrate, for example, a microarray chip or microplate, preferably suitable for use in high-throughput screening of biomolecules or cells. Substrates containing the fluorescent material also can be used to compensate for temperature variations in refractive index in optical sensors.