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Method for cloning and expression of SbfI restriction endonuclease and SbfI methylase in E. coli

机译：在大肠杆菌中克隆和表达SbfI限制性核酸内切酶和SbfI甲基化酶的方法

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摘要

The present invention relates to: recombinant DNA encoding the SbfI restriction endonuclease as well as the SbfI methylase, and expression of the SbfI restriction endonuclease and SbfI methylase in E. coli cells containing the recombinant DNA; and methods for cloning the SbfI restriction gene (sbfIR) from Streptomyces species Bf-61 into E. coli by PCR. The method relied on primers based on DNA sequences predicted from amino acid sequences of the purified SbfI restriction endonuclease.

机译：本发明涉及：编码 Sbf I限制性核酸内切酶以及 Sbf I甲基化酶的重组DNA，以及 Sbf I限制性酶的表达内切酶和 Sbf I甲基化酶。含有重组DNA的大肠杆菌细胞;链霉菌种Bf-61 中的 Sbf I限制性基因（ sbfIR ）克隆到 E中的方法和方法。 PCR 。该方法依靠基于从纯化的 Sbf I限制性核酸内切酶的氨基酸序列预测的DNA序列的引物。 展开▼

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公开/公告号EP1516927B1

专利类型

公开/公告日2007-10-24

原文格式PDF

申请/专利权人 NEW ENGLAND BIOLABS INC;
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申请/专利号EP20040077615

发明设计人 DAVIS THEODORE;LUNNEN KEITH;WILSON GEOFFREY;
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申请日2004-09-21

分类号C12N9/22;C12N15/55;

国家 EP

入库时间 2022-08-21 20:48:55

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3. A method for the cloning and expression of the restriction endonuclease sbfi and the methylase sbfi in e. coli [P] . 德国专利： DE602004009649T2 . 2008-08-14

机译：一种在大肠杆菌中克隆和表达限制性核酸内切酶sbfi和甲基化酶sbfi的方法。大肠杆菌

4. Method for cloning and expression of SbfI restriction endonuclease and SbfI methylase in E. coli [P] . 欧洲知识产权局专利： EP1516927A1 . 2005-03-23

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5. Method for cloning and expression of SbfI restriction endonuclease and SbfI methylase in E. coli [P] . 美国专利： US6958230B2 . 2005-10-25

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