ISOTOPICALLY LABELLED CROSSLINKING AGENTS FOR THE ANALYSIS OF PROTEIN INTERACTIONS

摘要

Agents and methods for qualitative and quantitative analysis a protein complex or protein complexes using isotope-labeled symmetrical bifunctional crosslinkers and mass spectrometry are provided. Targeting moieties, cell permeability moieties, or affinity moieties, may be appended to the bifunctional crosslinkers. The isotope-labeled symmetrical bifunctional crosslinkers may be used in a kit or as a library. The preferred crosslinker contains a cleavable core, e.g.