Recombinant Escherichia coli and overproduction method of polyhydroxybutyrate using the stain

摘要

The present invention relates to a recombinant recombinant Escherichia coli having a phbCAB gene derived from Alcaligenes eutrophus and a method for mass production of polyhydroxyalkanoate using the Escherichia coli, in more detail. is Alcaligenes oil Trojan to transfection prepare a E. coli transformed with a phbCAB gene Perth derived from a recombinant E. coli MG1655 / pTZ18U-PHB or JIL938 / pTZ18U-PHB, and an alkali to having phbCAB gene of Ness oil Trojan Perth derived step; Inoculating the recombinant E. coli in the medium and culturing the cells (cell growth period); Inducing polyhydroxyalkanoate production from the recombinant E. coli (cell stopper and product generator); And it relates to a mass production method of polyhydroxyalkanoate comprising inducing extracellular secretion of polyhydroxyalkanoate from the recombinant E. coli. The production method of the present invention not only enables mass production of polyhyalkanoate such as polyhydroxybutyrate by simple batch culture, but also simplifies the fermentation process and the separation and purification process, thereby replacing the existing hardly degradable synthetic plastics. It facilitates the industrial use of biodegradable polyhydroxyalkanoate.