A METHOD FOR ANALYZING METABOLITIC FLUX OF MICROORGANISM PRODUCING TRYPTOPHAN

摘要

The present invention to relates to a metabolic flux analysis method of the microorganism to produce tryptophan, separating the amino acids at more specifically cultured the tryptophan producing strain in a culture medium was added to the carbon atom probe isotopically culturing strains nuclear magnetic resonance spectrometry analysis and to a method for determining the flow rate in the important metabolic bio-strains, characterized in that for calculating the flow rate of tryptophan metabolism by the nuclear magnetic resonance analysis to analyze the metabolic characteristics of a tryptophan producing strain. ; According to the invention, the experimental method for NMR analyzing the probe isotopes in metabolites in determining the metabolic flux of the living body of a microorganism to produce tryptophan and, in theory, how parallel the genetic algorithm and the interior-reflective Newton method by applying the metabolic flux analysis technology by simultaneously it may provide a way to identify a more economic and quick tryptophan metabolism by analyzing the flow rate associated with the conventional analysis method than the tryptophan producing strain also directly metabolites accurately. ; Tryptophan, the microorganism is the nuclear magnetic resonance analysis method to produce tryptophan, TCA cycle