METHOD FOR REGENERATING SESAMUM INDICUM L

摘要

A method for regenerating Sesamum indicum L. is provided to induce an adventitious shoot from matured cotyledon of Sesamum indicum L. immediately before germination whose embryo is removed with high efficiency, so that a novel variety of Sesamum indicum L. having disease resistance, disaster resistance and high quality is developed. The method for regenerating Sesamum indicum L. comprises the steps of: absorbing water into matured seeds of Sesamum indicum L. immediately before the germination; culturing the cotyledon of Sesamum indicum L. whose testa and embryo are removed in MS medium containing 0.01-2.0 mg/l of IAA(indole-3-acetic acid), 0.01-7.0 mg/l of BA(6-benzyl-aminopurine), 0.01-2.0 mg/l of ABA(abscisic acid), 0.01-7 mg/l of AgNO3 and 1-9% sucrose for 2-3 weeks to induce calluses and adventitious shoots; transferring the induced calluses and adventitious shoots into MS medium containing 0.01-2.0 mg/l of IAA, 0.01-7.0 mg/l of BA, 0.01-2.0 mg/l of ABA, 0.01-7 mg/l of AgNO3 and 1-9% sucrose to induce multiple shoots; and subculturing the multiple shoots 2-3 times at an interval of 2 weeks.