MICROARRAY SUBSTRATE, METHOD OF ANALYZING BIOMOLECULE USING THE MICROARRAY SUBSTRATE, AND LAB-ON-A-CHIP INCLUDING THE MICROARRAY SUBSTRATE

摘要

A microarray substrate is provided to improve the fixation of a probe biomolecule on a substrate, decrease a non-specific binding of a protein to a target biomolecule on the substrate, and simultaneously perform the lysis process, PCR reaction, and hybridization of the probe and the target biomolecule. In addition, a method for analyzing a biomolecule using the same and a lab-on-a-chip comprising the same are provided. The microarray substrate is characterized in that a group represented by the formula(1) or (2) is fixed on a solid substrate. The method for analyzing a biomolecule comprises the steps of: (a) preparing a microarray substrate by fixing a group of the formula(1) or (2) on a solid substrate; (b) fixing a probe biomolecule on the microarray substrate under a first pH to prepare a microarray; (c) attaching a target biomolecule on the area other than the probe area of the microarray under the first pH; (d) under a second pH, after eluting the coupled target biomolecule, amplifying it; and (e) hybridizing the probe biomolecule on the microarray prepared by the step(b) with the target biomolecule. The lab-on-a-chip comprises the microarray substrate.