A COMPOSITION FOR DE-DIFFERENTIATING ASTROCYTES INTO NEURAL STEM CELL COMPRISING HUMAN BMI-1 PROTEIN OR A NUCLEIC ACID ENCODING AND THE SAME AND A PROCESS OF DE-DIFFERENTIATION USING SAID COMPOSITION

摘要

A composition comprising a Bmi-1 protein or a nucleic acid coding the Bmi-1 protein is provided to de-differentiate the astrocytes into neural stem cell-like cell which has the self-regeneration capability and the differentiation capability into astrocyte, neuron, and oligodendrocyte. The composition for inducing de-differentiation of astrocytes into neural stem cells comprises a Bmi-1 protein or a nucleic acid material including a nucleotide sequence coding a Bmi-1 protein, wherein the nucleic acid material containing a nucleotide sequence coding for a Bmi-1 protein is a vector allowing the Bmi-1 protein to be expressed. The method for inducing the de-differentiation of astrocytes comprises the steps of: (a) culturing astrocytes in a medium; (b) treating the astrocytes with the Bmi-1 protein or the nucleic acid material; and (c) inducing the astrocytes to differentiate into neural stem cells.