Characterization of AAV Structure: Capsid Proteins and Nucleic Acid Composition on LabChip GXII Touch Platform

摘要

For over fifty years, adeno-associated virus (AAV) has remained an elusive object in virology studies and gene therapeutics. Many biotherapeutic companies are currently involved in recombinant AAV production with needs of viral particle characterization in terms of capsid structure and nucleic acid content to estimate therapeutic doses.The PerkinElmer LabChip GXII Touch HT platform is an instrument to perform protein and nucleic acid analysis from the same sample with high sensitivity and reproducibility. The instrument can distinguish differences of protein molecular weight as low as 1kDa, which can be crucial to characterize artificially synthesized biopolymers such as recombinant capsid proteins. Additionally, the fast sample run times from multi-well plates allow for high throughput experiments and can open opportunities to monitor kinetic processes such as breakage of virus particles or their interactions with receptors.Extracted from multiple assays, our results show total analysis of all viral components after breaking apart of AAVs in various serotypes.