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PRIMER FOR DETECTING FOOD POISONING AND METHOD FOR RAPID DETECTION OF FOOD BORN PATHOGENE

机译:检测食物中毒的底漆和快速检测食物中病原菌的方法

摘要

The present invention relates to a primer for detecting food poisoning and a method for detecting bacterial food poisoning using the same. In particular, the present invention is a PCR primer capable of quickly and accurately amplifying specific genes of Salmonella spp., Staphylococcus aureus, Escherichia coli O157, Listeria monocytogenes and Vibrio parahemoliticus, and food poisoning detection using the primers. Methods and detection kits are provided. The food poisoning detection method of the present invention can detect food poisoning bacteria up to a concentration of 100 to 10 CFU / ml, and can perform rapid epidemiological investigation within 5 hours.;Food Poisoning, Nested PCR, Primer, Micro PCR
机译:本发明涉及一种用于检测食物中毒的引物和使用该引物的用于检测细菌性食物中毒的方法。特别地,本发明是一种PCR引物,其能够快速且准确地扩增沙门氏菌属,金黄色葡萄球菌,大肠杆菌O157,单核细胞增生性李斯特菌和副溶血性弧菌的特定基因,并使用该引物检测食物中毒。提供了方法和检测试剂盒。本发明的食物中毒检测方法可以检测到浓度高达100至10 CFU / ml的食物中毒细菌,并可以在5小时内进行流行病学调查。食物中毒,巢式PCR,引物,微型PCR

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