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Method for Detecting Rickettsia and scrub typhus groupSTG Using Real-time PCR

机译：实时荧光定量PCR检测立克次体和灌木斑疹伤寒组的方法

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摘要

The present invention relates to a method for the simultaneous detection of Rickettsia bacteria and Scrub typhoid group bacteria, and more specifically, the difference between the Rickettsia bacteria and the Tm values of specific fragments of the groEL gene amplified by real-time PCR. The present invention relates to a method for simultaneously detecting scrub typhoid group bacteria.;According to the present invention, the Rickettsia and Orientia tsutsugamushi can be detected more easily and more sensitively than any conventional DNA base test method.;Rickettsia, Cyber Green, Real-time PCR

机译：本发明涉及同时检测立克次体细菌和灌木型伤寒菌的方法，更具体地，涉及立克次体细菌与通过PCR扩增的 groEL 基因的特定片段的Tm值之间的差异。实时PCR。本发明涉及一种同时检测灌木型伤寒菌的方法。根据本发明，与任何常规的DNA碱基检测方法相比，立克次体和虫东方Or可以更容易地检测。 ;立克次体，Cyber Green，实时PCR 展开▼

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公开/公告号KR100681837B1

专利类型

公开/公告日2007-02-12

原文格式PDF

申请/专利权人 건국대학교 산학협력단;
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申请/专利号KR20050049466

发明设计人 이승현;박효순;이정희;정은주;
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申请日2005-06-09

分类号C12Q1/68;

国家 KR

入库时间 2022-08-21 20:32:55

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1. 莫氏立克次体及恙虫病东方体双重实时荧光定量PCR检测体系及检测方法 [P] . 中国专利： CN105018610B . 2019.02.15

2. 莫氏立克次体及恙虫病东方体双重实时荧光定量PCR检测体系及检测方法 [P] . 中国专利： CN105018610A . 2015-11-04

3. Method for Detecting Rickettsia and scrub typhus groupSTG [P] . 韩国专利： KR100681820B1 . 2007-02-12

机译：立克次体和斑疹伤寒组的检测方法

4. METHOD FOR DETECTING RICKETTSIA AND SCRUB TYPHUS GROUP(STG) USING REAL-TIME PCR [P] . 韩国专利： KR20060128217A . 2006-12-14

机译：实时荧光定量PCR检测立克次体和小SCR科的方法

5. METHOD FOR DETECTING RICKETTSIA AND SCRUB TYPHUS GROUP(STG) [P] . 韩国专利： KR20060128201A . 2006-12-14

机译：检测立克次体和小SCR蒲组的方法

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2. Establishment of a Real-time Fluorescent Quantitative RTPCR Method for Detecting NP Gene of Class Ⅰ Newcastle Disease Virus(NDV) [J] . Junping CAO, Xiaoquan WANG, Han CHENG, 农业生物技术：英文版 . 2018,第006期

3. Application of a real-time PCR method for detecting and monitoring hookworm Necator americanus infections in Southern China [J] . Jia-Xu Wang, Cang-Sang Pan, Li-Wang Cui 亚太热带生物医学杂志（英文版） . 2012,第012期

4. Application of a real-time PCR method for detecting and monitoring hookworm Necator americanus infections in Southern China [J] . Jia-Xu Wang, Cang-Sang Pan, Li-Wang Cui 亚太热带生物医学杂志：英文版 . 2012,第012期

5. Development and Preliminary Application of SYBR Green I Real-Time Fluorescence Quantitative PCR Method for Detecting Porcine Parvovirus Virus [J] . SHEN Zhi-qiang, WANG Jin-liang, GUO Xian-po, 动物与饲料科学：英文版 . 2009,第011期

6. Non-parametric MANOVA Methods for Detecting Differentially Expressed Genes in Real-Time RT-PCR Experiments [C] . Niccolo Bassani, Federico Ambrogi, Roberta Bosotti, International Meeting on Computational Intelligence Methods for Bioinformatics and Biostatistics . 2010

7. Ecology of scrub typhus in Taiwan: The impact of environment, rodent community, and socio-economy. [D] . Kuo, Chi-Chien. 2010

8. Use of Multiplex Real-Time PCR To Diagnose Scrub Typhus [O] . Wiwit Tantibhedhyangkul, Ekkarat Wongsawat, Saowaluk Silpasakorn, 2017

9. Development of a duplex real-time PCR for the detection of Rickettsia spp. and typhus group rickettsia in clinical samples [O] . Giulieri, Stefano, Jaton, Katia, Cometta, Alain, 2011

10. Sensitive Microplate Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the Scrub Typhus Rickettsia, Rickettsia Tsutsugamushi. [R] . Dasch, G. A., Halle, S., Bourgeois, A. L. 1978

1. 检测斑疹伤寒病原体的实时荧光定量PCR方法建立 [C] . 杨晓 ,陈梅玲 ,温博海 . 第九届全军流行病学、第八届全军防生物危害医学专业学术会议 . 2007

2. 检测斑疹伤寒病原体实时荧光定量PCR方法的建立黑龙江立克次体和立克次体精河株毒力和抗原性初步研究 [A] . 杨晓 . 2007