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Freezing preservation method of ark-shell larva
Freezing preservation method of ark-shell larva
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机译:方舟幼虫的冷冻保存方法
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摘要
A freezing preservation method of ark-shell larva is provided, thereby systematically producing the ark-shell larva by preserving seeds of ark-shell larva, and reducing works for maintenance of parent ark-shell. The freezing preservation method of ark-shell larva comprises the steps of: inserting the ark-shell larva into the synthetic seawater containing cryoprotectants; storing the ark-shell larva absorbing cryoprotectants at room temperature for the equilibrium time; and freezing the stored ark-shell larva using a freezer, wherein the cryoprotectant is selected from dimethyl sulfoxide, glycerol, ethylene glycol, propylene glycol and methanol; the seawater contains 1.5 to 2 M of cryoprotectants; and the equilibrium time is 10 to 20 minutes.
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