. The primer set for specific DNA segments detection of Xanthomonas campestris pv. vesicatoria by PCR method and the detecting method thereof

摘要

The present invention relates to a hospital-type bacterial pathogen spots (Pathovar) specific PCR primer sets that can be quickly and accurately detect the specific DNA fragment of (Xanthomonas campestris pv. Vesicatoria). More specifically, the primer set of the present invention is a pair of primer sets made of 20mer which detects only bacterial spot pattern pathogens by using a polymerase chain reaction (PCR) method, and the primer set is a bacterial spot pattern pathogen ( Xanthomonas campestris pv). vesicatoria alone forms a single band of 517 bp and does not detect bands in similar species and other pathogenic strains in other Xanthomonas genus, and rapidly and precisely on the DNA level within 6 hours, Xanthomonas campestrispv.vesicatoria ), It is possible to determine the authenticity of the infection, which is effective in predicting the seed infection and development in the cultivation field.;Bacterial spotted pathogen, Xanthomonas campestris pv. vesicatoria, specific primers, early diagnosis of pathogens, Rhs