. The primer set for specific DNA segments detection of Xanthomonas campestris pv. vesicatoria by PCR method and the detecting method thereof

摘要

The invention spots bacterial pathogens ( Xanthomonas campestris pv. vesicatoria ) quickly a specific DNA fragment, and precisely relates to a hospital-type (Pathovar) specific PCR primer sets that can be detected. In a more specifically, the pair of primer sets designed to detect only the 20mer that bacterial pathogens spots primer set using the PCR (polymerase chain reaction) method of the present invention, the primer set is a bacterial pathogen spots ( Xanthomonas campestris pv. vesicatoria ) only form a single band of 517bp and other Santorini Thira ( Xanthomonas ) in my similar species and other pathogenic strain has detected a band not, at the DNA level within 6 hours of fast, accurate bacterial pathogens spots (. Xanthomonas campestris pv vesicatoria ), and whether it is possible to determine whether seed cultivation infection authenticity of infection has an effect on the generation surveillance in the packaging.