A METHOD FOR NORMAL EMBRYO PRODUCTION THROUGH MICROSPORE CULTURE IN CAPSICUM ANNUUM L

摘要

A method for production of normal embryo of red pepper(Capsicum annuum L.) through microspore culture is provided to improve production yield of normal embryo of red pepper(Capsicum annuum L.) in the microspore culture by using the microspore as a pretreating material and performing double layer culture. The method for production of normal embryo of red pepper(Capsicum annuum L.) through microspore culture comprises the steps of: blending and vortexing the anther collected from the flower bud of red pepper(Capsicum annuum L.) to obtain microspores; removing somatic cell tissue fragments from the obtained microspores, and centrifuging them to obtain microspores; pretreating the microspores in a pretreatment medium and culturing at 32 deg.C for 3 days; culturing the pretreated microspores on NLN medium in a density of 80,000-100,000 microspores/ml for 1 day, adding fresh NLN medium into the microspores, and culturing them at 27 deg.C under dark condition for 3-4 weeks, wherein the pretreatment medium is NLN(sucrose excluded) medium containing 0.37M mannitol.