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THE CULTIVATING METHOD OF LENTINUS EDODES CONTAINING HIGH CONTENT OF SELENIUM USING ASPARTIC ACID

机译:用乙酸培育高含量硒香菇的方法。

摘要

A method of cultivating shiitake mushroom(oak mushroom, Lentinus edodes) and white shiitake mushroom using a medium or soaking liquid containing selenium and aspartic acid to induce accumulation of the selenium is provided, which cultivates shiitake mushroom having high contents of selenium, provides optimal temperature and humidity conditions for inducing white shiitake mushroom and recycles used medium. The shiitake mushroom is cultivated by the steps of: culturing spawn in a liquid medium for 2 to 3 days; adding 0.01 to 0.05ppm selenite solutions and 5 to 10ppm aspartic acid to the liquid medium and performing spawn culturing; inoculating spawn cultures into a solid medium containing 0.01 to 0.05ppm selenium and 5 to 10ppm aspartic acid and culturing until the diameter of mushroom cap reaches 2 to 4cm; and soaking the solid medium remaining after harvesting in soaking liquid containing 0.05 to 0.1ppm inorganic selenium and 10 to 20ppm aspartic acid for 16 to 24hr to give a medium with a moisture content of at least 75%. The medium is recycled 1 to 7 times. The white shiitake mushroom is cultivated by the steps of: cultivating shiitake mushroom with a cap size of 2 to 4cm while maintaining the internal temperature and humidity at 10±3deg.C and 75±5%; quickly changing the internal temperature and humidity to 35 to 40deg.C and 35 to 40% for 2 to 4hr using hot air to induce separation of the cap surface; quickly reducing to 5 to 10deg.C and 70 to 80%; and continuing the above step 1 to 3 times per day for 7 days to cause a change in color from brown to white.
机译:本发明提供了一种利用含硒和天冬氨酸的培养基或浸泡液培养香菇的方法,该方法可以培育出硒含量高的香菇,并提供适宜的温度。和湿度条件诱导白香菇并回收使用过的培养基。香菇的栽培步骤如下:将卵在液体培养基中培养2至3天。向液体培养基中添加0.01至0.05ppm的亚硒酸盐溶液和5至10ppm的天冬氨酸并进行产卵培养。将产卵培养物接种到含有0.01至0.05ppm的硒和5至10ppm的天冬氨酸的固体培养基中,并进行培养直至蘑菇帽的直径达到2至4厘米。将收获后剩余的固体培养基在含有0.05至0.1ppm的无机硒和10至20ppm的天冬氨酸的浸泡液中浸泡16至24小时,以得到水分含量至少为75%的培养基。介质被回收1至7次。通过以下步骤来栽培白香菇:在保持内部温度和湿度在10±3℃和75±5%的条件下,培养2至4cm大小的香菇。使用热空气将内部温度和湿度快速更改为35至40摄氏度和35至40%,持续2至4个小时,以引起瓶盖表面分离;快速降低至5至10摄氏度和70至80%;并每天继续执行上述步骤1至3次,共7天,以使颜色从棕色变为白色。

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