NOVEL IMINECALIXARENE DERIVATIVES METHOD OF PREPARATION THEREOF AND SELF-ASSEMBLED MONOLAYER PREPARED BY THE METHOD FIXING METHOD OF OLIGO-DNA BY USING THE SELF-ASSEMBLED MONOLAYER AND OLIGO-DNA CHIP PREPARED BY THE METHOD

摘要

This invention relates to the formula (1) and (2) novel immigration Cal Riggs arene derivatives and their preparation methods, and oligonucleotide utilizing a self-assembled monolayer, the self-assembled monomolecular layer produced by the above production method oligonucleotides prepared by DNA immobilization method and thus It relates to a DNA chip. ; [Formula 1] ; (In the above formula, R _1, R _2, R ₃ and R ₄ are each independently _{-H, -CH 3, -C 2 H 5} , -C 3 H 7, -OCH 3, -Cl, -C 6 H _5, and are selected from the group consisting of -COOR, R in the -COOR represents a -CH ₃ or -C ₂ H _5.); [Formula 2] ; (In the above formula, R _1, R _2, R ₃ and R ₄ are each independently _{-H, -CH 3, -C 2 H 5} , -C 3 H 7, -OCH 3, -Cl, -C 6 H _5, and are selected from the group consisting of -COOR, it represents a -COOR wherein R is in -CH ₃ or -C ₂ H _5. in addition, the _Y 1, Y 2, Y 3 and Y ₄ are each independently -H, _{- (CH 2) n-CH} = O, - (CH 2) n-SH, - (CH 2 CH 2 O) m-CH 2 CH 2-CH = O, - (CH 2 CH 2 O) m-CH a _{(CH 2) c -Z - 2} CH 2 -SH, - (CH 2) m -C 6 H 4 - (CH 2) c -Z and -CO- (CH _2)m-1 -C ₆ H ₄ is selected from the group consisting of (defined as n = 2 ~ 15, m = 1 ~ 10, c = 0 ~ 10, Z = -SH, -CHO, -COOH or -NH _2).); Immigration Cal Riggs Arendal derivatives, a self-assembled monolayer solid substrate, oligo DNA chip