首页> 外国专利> METHOD FOR ANALYSING SINGLE NUCLEOTIDE POLYMORPHISMS OF GERIATRIC DISEASES USING OLIGONUCLEOTIDE CHIP AND KIT THEREFOR

METHOD FOR ANALYSING SINGLE NUCLEOTIDE POLYMORPHISMS OF GERIATRIC DISEASES USING OLIGONUCLEOTIDE CHIP AND KIT THEREFOR

机译:寡核苷酸芯片及其试剂盒分析老年性疾病的单核苷酸多态性的方法

摘要

The invention hypertension, atherosclerosis, dementia, osteoporosis, single nucleotide polymorphisms existing in the adult disease-related genes, such as diabetic complications (Single Nucleotide Polymorphisms: hereinafter "SNPs" abbreviated as) test method and a test kit to be used therefor It relates. ; The present invention is the polymerase chain reaction of the gene to be examined: the step of amplifying using an (Polymerase Chain Reaction hereinafter "PCR"); Oligonucleotide containing the SNPs present in the adult disease related genes comprising oligonucleotides attached to the base is attached using the amplification product hybridization (hybridization) of the base chip; And inspection by using the SNPs inspection method comprising the steps to determine specifically bound SNPs in the hybridization reaction, rapid each adult SNPs of the relevant gene, accurately, and also checks the number of SNPs in the gene with a single scan of the same time can do. ; Adult diseases, gene polymorphism, examination methods, test kits.
机译:本发明的高血压,动脉粥样硬化,痴呆,骨质疏松症,与成人疾病相关的基因中存在的单核苷酸多态性,例如糖尿病并发症(单核苷酸多态性:以下简称为“ SNP”)测试方法及其测试盒涉及。 ;本发明是要检查的基因的聚合酶链反应:使用(以下称为“ PCR”的聚合酶链反应)进行扩增的步骤;以及使用以下的方法进行扩增的步骤。使用基础芯片的扩增产物杂交(杂交)来连接包含存在于与成年人疾病相关的基因中的SNP的寡核苷酸,所述SNP包含与碱基相连的寡核苷酸。并且,通过使用SNP检查方法进行检查,该方法包括以下步骤:确定杂交反应中特异性结合的SNP,准确地快速筛选相关基因的每个成年SNP,并且同时进行一次扫描来检查基因中SNP的数量可以做。 ;成人疾病,基因多态性,检查方法,检测试剂盒。

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