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METHOD OF REAL TIME DETECTION OF PATHOGENIC MICROORGANISM USING THE COMPETITIVE IMMUNOREACTION-MEDIATED FLOW TYPE SURFACE PLASMON RESONANCE BIOSENSOR
METHOD OF REAL TIME DETECTION OF PATHOGENIC MICROORGANISM USING THE COMPETITIVE IMMUNOREACTION-MEDIATED FLOW TYPE SURFACE PLASMON RESONANCE BIOSENSOR
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机译:竞争性免疫介导的流型表面等离子体共振生物传感器实时检测病原微生物的方法
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摘要
A method for real time detection of pathogenic bacteria by using a competitive immunoreaction-mediated flow type surface plasmon resonance(SPR) biosensor is provided to improve ability for detecting low concentration of waterborne pathogenic bacteria including Cryptosporidium parvum without labeling. A method for real time detection of pathogenic bacteria comprises the steps of: (i) immunoreacting a pathogenic bacterium with antibodies thereof(first antibody) in a batch type; (ii) selectively separating unreacted antibodies by using cut-off membrane filter; (iii) binding the separated unreacted antibodies to a second antibody immobilized to the surface of a flow type surface plasmon resonance(SPR) system chip in real time; and (iv) continuously collecting the real time SPR signal generated from the surface of flow type SPR system chip of unreacted antibody.
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