GENES CORYNEBACTERIUM GLUTAMICUM, encode proteins metabolic pathways

摘要

1. u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 nucleic acid molecule Corynebacterium glutamicum, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 sequence selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of sequences with odd numbers mi SEQ id no: 3 - 1155, provided that nucleic acid molecule is not any of the f - designated genes represented in table 1, or u043au043eu043cu043fu043bu0435u043cu0435u043du0442u0430u0440u043du0443u044e her after u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u044c.;2. u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 nucleic acid molecule, u043au043eu0442u043eu0440u0430u00a0 encodes the polypeptide containing the u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of u043fu043eu0441u043bu0435u0434u043eu0432u0430 u0442u0435u043bu044cu043du043eu0441u0442u0435u0439 with even numbers SEQ id no: 4 - 1156, provided that nucleic acid molecule is not no one f - designated genes represented in table 1, or u043au043eu043cu043fu043bu0435u043cu0435u043du0442u0430u0440u043du0443u044e her sequence.;3.u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 nucleic acid molecule, u043au043eu0442u043eu0440u0430u00a0 encodes natural u0430u043bu043bu0435u043bu044cu043du044bu0439 option u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 comprising u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence selected from group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 sequences with even numbers SEQ id no: 4 - 1156, provided that nucleic acid molecule is not any of the f - designated genes table 1 or u043au043eu043cu043fu043bu0435u043cu0435u043du0442u0430u0440u043du0443u044e her sequence.;4. u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 nucleic acid molecule, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 u043du0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e sequence u043au043eu0442u043eu0440u0430u00a0 by at least 50% identical to the u043du0443u043au043bu0435u043eu0442u0438u0434u043du043eu0439 u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441 t any sequence with an odd number SEQ id no: 3 - 1155, provided that nucleic acid molecule is not any of the f - defined genes, given by u043du043du044bu0445 in table 1, or u043au043eu043cu043fu043bu0435u043cu0435u043du0442u0430u0440u043du0443u044e her sequence.;5. u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 nucleic acid molecule, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 fragment, u0441u043eu0441u0442u043eu00a0u0449u0438u0439 of at least 15 consecutive nucleotide sequence u043du0443u043au043bu0435u043eu0442u0438u0434u043du043eu0439 love in consistency with an odd number SEQ id no: 3 - 1155, provided that nucleic acid molecule is not any of the f - designated genes presented in t u0430u0431u043bu0438u0446u0435 1 or u043au043eu043cu043fu043bu0435u043cu0435u043du0442u0430u0440u043du0443u044e her sequence.;6. u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 nucleic acid molecule, a nucleic acid molecule u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 on any of the u043fu043f.1 - 5, and u043du0443u043au043bu0435u043eu0442u0438u0434u043du0430u00a0 sequence u043au043eu0434u0438u0440u0443u044eu0449u0430u00a0 u0433u0435u0442u0435u0440u043eu043bu043eu0433u0438u0447u043du044b s polypeptide.;7. a vector containing the nucleic acid molecule for any of the u043fu043f.1 - 6.;8. vector on p.7, which u00a0u0432u043bu00a0u0435u0442u0441u00a0 u044du043au0441u043fu0440u0435u0441u0441u0438u0440u0443u044eu0449u0438u043c vector.;9. the cell u0445u043eu0437u00a0u0438u043d, u0442u0440u0430u043du0441u0444u0435u0446u0438u0440u043eu0432u0430u043du043du0430u00a0 u044du043au0441u043fu0440u0435u0441u0441u0438u0440u0443u044eu0449u0438u043c vector at point 9.;10. the cell u0445u043eu0437u00a0u0438u043d on p.9, where u0443u043au0430u0437u0430u043du043du0430u00a0 cell u00a0u0432u043bu00a0u0435u0442u0441u00a0 u043cu0438u043au0440u043eu043eu0440u0433u0430u043du0438u0437u043cu043eu043c.;11. the cell u0445u043eu0437u00a0u0438u043d on p.10, where u0443u043au0430u0437u0430u043du043du0430u00a0 cell belongs to the family of Corynebacterium or Brevibacterium.;12. the cell u0445u043eu0437u00a0u0438u043d on u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 p.9, where the nucleic acid molecule leads to the u043cu043eu0434u0443u043bu00a0u0446u0438u0438 u043fu0440u043eu0434u0443u0446u0438u0440u043eu0432u0430u043du0438u00a0 chemical product thin organic sinta..,.. for out of the cells.;13. the cell u0445u043eu0437u00a0u0438u043d on p.12, where the chemical product of thin organic synthesis is selected from the group consisting of organic acid, u043fu0440u043eu0442u0435u0438u043du043eu0433u0435u043du043du044bu0435 and u043du0435u043fu0440u043eu0442u0435u0438 u043du043eu0433u0435u043du043du044bu0435 amino acids, u043fu0443u0440u0438u043du043eu0432u044bu0435 and u043fu0438u0440u0438u043cu0438u0434u0438u043du043eu0432u044bu0435 u043eu0441u043du043eu0432u0430u043du0438u00a0, nucleoside, nucleotide, lipid, saturated and unsaturated fatty acids, u0434u0438u043eu043bu044b, carbohydrates, u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441 among u0441u043eu0435u0434u0438u043du0435u043du0438u00a0, vitamins, u043au043eu0444u0430u043au0442u043eu0440u044b, polyketide, and enzymes.;14. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430, including cultivation of cells is u0445u043eu0437u00a0u0438u043du0430 on p.9 in u043fu043eu0434u0445u043eu0434u00a0u0449u0435u0439 u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 environment u0434u043bu00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 through the u043fu043eu043bu0438u043fu0435u043fu0442 ida.;15. the polypeptide containing the u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence selected from the group, u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of sequences with even numbers SEQ id no: 4 - 1156, rm3 u043eu0432u0438u0438 that u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0430u00a0 sequence not u043au043eu0434u0438u0440u0443u0435u0442u0441u00a0 any of the f - designated genes presented in table 1.;16. the polypeptide containing the natural u0430u043bu043bu0435u043bu044cu043du044bu0439 option u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 containing u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of pefc u0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u0435u0439 with even numbers SEQ id no: 4 - 1156, provided that u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0430u00a0 sequence not u043au043eu0434u0438u0440u0443u0435u0442u0441u00a0 any of the f - designated genes presented in t u0430u0431u043bu0438u0446u0435 1.;17.the polypeptide, which u043au043eu0434u0438u0440u0443u0435u0442u0441u00a0 nucleic acid molecule containing u043du0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e sequence u043au043eu0442u043eu0440u0430u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 by at least 50 per cent of eden the sequence of nucleic acid u0442u0438u0447u043du0430 any sequence with an odd number SEQ id no: 3 - 1155, provided that not u0441u043eu0434u0435u0440u0436 nucleic acid molecule it is of the f - marked nucleic acid molecules.presented in table 1.;18. the polypeptide containing the u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence u043au043eu0442u043eu0440u0430u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 by at least 50% identical to the sequence u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du043eu0439 any p u043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u0438 with an even number SEQ id no: 4 - 1156, provided that the u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0430u00a0 sequence not u043au043eu0434u0438u0440u0443u0435u0442u0441u00a0 any of the f - designated genes presented x in the table 1.;19.the polypeptide containing the u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 containing u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence of any sequence with an even number SEQ id no: 4 - 1156, in provided that the u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0430u00a0 sequence not u043au043eu0434u0438u0440u0443u0435u0442u0441u00a0 any of the f - designated genes represented in table 1, where the u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u043du044bu0439 u0444u0440u0430u0433u043cu0435u043d t u0441u043eu0445u0440u0430u043du00a0u0435u0442 biological activity u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430,a u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence.;20. the selected polypeptide on any of the u043fu043f.15 - 19, further containing u0433u0435u0442u0435u0440u043eu043bu043eu0433u0438u0447u043du044bu0435 u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du044bu0435 sequence.;21. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 chemical product, fine organic synthesis, including the cultivation of cells on the p.9, so u043fu0440u043eu0434u0443u0446u0438u0440u0443u0435u0442u0441u00a0 fine chemical product organic synthesis.;22. way on the way p.21, where further includes phase u0438u0437u0432u043bu0435u0447u0435u043du0438u00a0 thin organic synthesis chemical product from the culture.;23. method for u0443u043au0430u0437u0430u043du043du0430u00a0 p.21, where a belongs to the family of Corynebacterium or Brevibacterium.;24. method for p.21, where u0443u043au0430u0437u0430u043du043du0430u00a0 cell selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of Corynebacterium glutamicum, Corynebacterium herculis, Corynebacterium lilium, Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Corynebacterium acetophilum, Corynebacterium ammoniag enes, Corynebacterium fujiokense, Corynebacterium nitrilophilus, Brevibacterium ammoniagenes, Brevibacterium butanicum,Brevibacterium divaricatum, Brevibacterium flavum, Brevibacterium healii, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum, Brevibacterium lactofermentum, Brevibacterium sea Brevibacterium paraffinolyticum and these strains reported in table 3.;25. method for u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 p.21, where the nucleic acid molecules of the vector leads to the thin org u043cu043eu0434u0443u043bu00a0u0446u0438u0438 u043fu0440u043eu0434u0443u0446u0438u0440u043eu0432u0430u043du0438u00a0 chemical product u0430u043du0438u0447u0435u0441u043au043eu0433u043e synthesis.;26. method for p.21, where the chemical product of thin organic synthesis is selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 from organic acids, u043fu0440u043eu0442u0435u0438u043du043eu0433u0435u043du043du044bu0445 and u043du0435u043fu0440u043eu0442u0435u0438u043du043eu0433u0435u043du043d s amino acids, u043fu0443u0440u0438u043du043eu0432u044bu0445 and u043fu0438u0440u0438u043cu0438u0434u0438u043du043eu0432u044bu0445 grounds, u043du0443u043au043bu0435u043eu0437u0438u0434u043eu0432, nucleotides, lipids, saturated and unsaturated fatty acids, u0434u0438u043eu043bu043eu0432, carbohydrate, aromatic compounds, vitamins, u043au043eu0444u0430u043au0442u043eu0440u043eu0432, u043fu043eu043bu0438u043au0435u0442u0438u0434u043eu0432 and enzymes.;27. method for p.21, where the chemical product u00a0u0432u043bu00a0u0435u0442u0441u00a0 u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043eu0439.;28. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.27, so that u0443u043au0430u0437u0430u043du043du0430u00a0 amino acid selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of lysine, u0433u043bu0443u0442u0430u043cu0430u0442u0430, u0433u043bu0443u0442u0430u043cu0438u043du0430, u0430u043bu0430u043du0438u043du0430, u0430u0441u043fu0430u0440u0442u0430u0442u0430, u0433u043bu0438u0446u0438u043du0430, serina, u0442u0440u0435u043eu043du0438 on, methionine, u0446u0438u0441u0442u0435u0438u043du0430, valin, u043bu0435u0439u0446u0438u043du0430, u0438u0437u043eu043bu0435u0439u0446u0438u043du0430, u0430u0440u0433u0438u043du0438u043du0430, u043fu0440u043eu043bu0438u043du0430, u0433u0438u0441u0442u0438u0434u0438u043du0430, u0442u0438u0440u043eu0437u0438u043du0430, u0444u0435u043du0438u043bu0430u043bu0430u043du0438u043du0430 and tryptophan.;29. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 chemical product, fine organic synthesis, including the cultivation of cells, which was modified by the inclusion of u0433u0435u043du043eu043cu043du0430u00a0 dna molecules nucleic acid on any of the u043fu043f.1 - 6.;30. method of diagnosing u043fu0440u0438u0441u0443u0442u0441u0442u0432u0438u00a0 or Corynebacterium diphtheriae activity in the subject, including the detection of single molecules u043fu0440u0438u0441u0443u0442u0441u0442u0432u0438u00a0 nucleic acid on u043fu043f.1 - 5 or p u043eu043bu0438u043fu0435u043fu0442u0438u0434u043du044bu0445 molecules on u043fu043f.15 - 19, and thus the diagnosis u043fu0440u0438u0441u0443u0442u0441u0442u0432u0438u00a0 Corynebacterium diphtheriae or activity in the subject.;31. the cell u0445u043eu0437u00a0u0438u043d, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 nucleic acid molecule selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of sequences with odd numbers SEQ id no: 3 - 1155, where the molecule nook u043bu0435u0438u043du043eu0432u043eu0439 acid u00a0u0432u043bu00a0u0435u0442u0441u00a0 destroyed.;32. the cell u0445u043eu0437u00a0u0438u043d, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 nucleic acid molecule selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of sequences with odd numbers SEQ id no: 3 - 1155, where u043du0443u043au043bu0435u0438 molecule a new acid contains one or more modifications of nucleic acids compared with u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u00a0u043cu0438 with odd numbers SEQ id no: 3 - 1155;33. the cell u0445u043eu0437u00a0u0438u043d, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 nucleic acid molecule selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of sequences with odd numbers SEQ id no: 3 - 1155, where u0440u0435u0433u0443u043bu00a0u0442u043eu0440u043du044bu0439 ouch the nucleic acid molecules u0430u0441u0442u043eu043a modified on the u0440u0435u0433u0443u043bu00a0u0442u043eu0440u043du043eu0433u043e station that molecules of wild type.