Strain Escherichia coli BL21 (DE3) pAYC-ET- (hIFN-ALFA2b) -lacI - PRODUCER recombinant human interferon alpha-2b and method for its cultivation

摘要

1. strain of bacteria escherichia coli BL21 (DE3) [pAYC - et - (hIFN - 2b) - lacI] - a producer with u043bu0435u0439u043au043eu0446u0438u0442u0430u0440u043du043eu0433u043e alpha 2b interferon.;2.method u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0 bacteria escherichia coli in the environment m 9 containing glucose, u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 u043au0430u0437u0435u0438u043du0430, antibiotic and mineral supplements, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 the fish pieces u bacteria escherichia coli BL21 (DE3) [pAYC - et - (hIFN - 2b) - lacI], u043fu0438u0442u0430u0442u0435u043bu044cu043du0430u00a0 environment contains 10 to 20 g u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u0430 u043au0430u0437u0435u0438u043du0430 and as an antibiotic, streptomycin in a dose of 20 to 40 mg / la further 5 to 10 g / l of barmy of extract and 0.05 - 0.1 pills of vitamins and mineral substance "centrum" and the cultivation of u043fu0440u043eu0432u043eu0434u00a0u0442 introduction environment industrialization u043au0442u043eu0440u0430 interferon while maintaining u043fu043eu0441u0442u043eu00a0u043du043du043eu0439 concentration of glucose in the environment at the level of 0.01 - 0.03 g / l, and the ph of 6.7 and 6.8.;3. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 2, so that a u0438u043du0434u0443u043au0442u043eu0440u0430 interferon u0432u0432u043eu0434u00a0u0442 1.2 - u0438u0437u043eu043fu0440u043eu043fu0438u043b - tio - beta u0433u0430u043bu0430u043au0442u043eu0437u0438u0434 in dose of 0.1 to 0.2 g / l.;4. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 2, so that a u0438u043du0434u0443u043au0442u043eu0440u0430 u0432u0432u043eu0434u00a0u0442 interferon intolerant in doses of 6 to 8 g / l.;5. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 2, so that the regulation of ph and glucose after u0441u043eu0434u0435u0440u0436u0430u043du0438u00a0 u0438u043du0434u0443u0446u0438u0440u043eu0432u0430u043du0438u00a0 u043fu0440u043eu0438u0437u0432u043eu0434u00a0u0442 introduction in an environment u0433u043bu044eu043au043eu0437u043eu0441u043eu0434u0435u0440u0436u0430u0449u0435u0439 additives (volume of 100 ml to 1 l environment u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0), which is composed of the following ingredients: mas.;acid u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 u043au0430u0437u0435u0438u043du043020 - 25u0434u0440u043eu0436u0436u0435u0432u043eu0439 u044du043au0441u0442u0440u0430u043au044210 - 15u0433u043bu044eu043au043eu0437u043020 - 50u043cu0430u0433u043du0438u0439 sulphite u0441u0435u043cu0438u0432u043eu0434u043du044bu04390,1 - 0.6