Antigen-specific way certain complex alpha-2-macroglobulin With cytokines in blood serum

摘要

antigen specific way u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 complexes of alpha 2 - u043cu0430u043au0440u043eu0433u043bu043eu0431u0443u043bu0438u043du0430 with u0446u0438u0442u043eu043au0438u043du0430u043cu0438 in blood serum using immune enzyme analysis, u0432u043au043bu044eu0447u0430u044eu0449u0438 in u0441u0432u00a0u0437u044bu0432u0430u043du0438u0435 complexes, u043du0430u0445u043eu0434u00a0u0449u0438u0445u0441u00a0 in blood serum with the primary u0430u0444u0444u0438u043du043du043e - u043eu0447u0438u0449u0435u043du043du044bu043cu0438 u043fu043eu043bu0438u043au043bu043eu043du0430u043bu044cu043du044bu043cu0438 rabbit trail antibodies against alpha 2 - u043cu0430u043au0440u043eu0433u043bu043eu0431u0443u043bu0438u043du0430. the century on the surface of polystyrene and, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 orderwhat as secondary antibodies used u0431u0438u043eu0442u0438u043du0438u043bu0438u0440u043eu0432u0430u043du043du044bu0435 monoclonal antibodies to studied in complex with alpha (2) - u043cu0430u043au0440u043eu0433u043bu043eu0431u0443u043bu0438u043du043eu043c u0446u0438u0442u043eu043au0438u043du0443, as the indicator component is u043au043eu043du044cu044eu0433u0430u0442 u0441u0442u0440u0435u043fu0442u0430u0432u0438u0434u0438u043du0430 with u043fu0435u0440u043eu043au0441u0438u0434u0430u0437u043eu0439 fuck, the reaction mixture is u043fu0440u043eu00a0u0432u043bu00a0u044eu0442 substrate u043d2u043e2 and u043au0440u0430u0441u0438u0442u0435u043bu00a0 3.3 5.5 u0442u0435u0442u0440u0430u043cu0435u0442u0438u043bu0431u0435u043du0437u0438u0434u0438u043du0430, watching the development of blue dye, reaction stopping 1N sulphuric acidu043eu043fu0440u0435u0434u0435u043bu00a0u044eu0442 optical density in the investigated samples and u043du0430u0445u043eu0434u00a0u0442 concentration u0446u0438u0442u043eu043au0438u043du0430 complexes with alpha 2 - u043cu0430u043au0440u043eu0433u043bu043eu0431u0443u043bu0438u043du043eu043c on the calibration curve, u0434u043bu00a0 u043fu043eu0441u0442u0440u043eu0435 u043du0438u00a0 which as primary antibodies using monoclonal antibodies to the independent u044du043fu0438u0442u043eu043fu0443 molecules u0446u0438u0442u043eu043au0438u043du0430,as the session, several samples of solutions with known u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0u043cu0438 u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0433u043e u0446u0438u0442u043eu043au0438u043du0430 in buffer u0434u043bu00a0 u0440u0430u0437u0432u0435u0434u0435u043du0438u00a0, as secondary antibodies - u0431u0438u043eu0442u0438u043du0438u043bu0438u0440u043eu0432u0430u043du043du044bu0435 monoclonal antibodies to independent u044du043fu0438u0442u043eu043fu0430u043c molecules u0446u0438u0442u043eu043au0438u043du0430, as a tracer component u043au043eu043du044cu044eu0433u0430u0442 u0441u0442u0440u0435u043fu0442u0430u0432u0438u0434u0438u043du0430 with u043fu0435u0440u043eu043au0441u0438u0434u0430u0437 oh shit, the reaction mixture u043fu0440u043eu00a0u0432u043bu00a0u044eu0442 by substrate - H2O2 and u043au0440u0430u0441u0438u0442u0435u043bu00a0 - 33 5.5 u0442u0435u0442u0440u0430u043cu0435u0442u0438u043bu0431u0435u043du0437u0438u0434u0438u043du0430, watching the blue dye, reaction stopping 1N sulphuric acid, u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0442 optical density in the sample session) u0440u043eu00a0u0442 calibration curve.