METHOD FOR DETECTING CONCENTRATION OF ANTIBODIES IN BLOOD SERA TO PATHOGENIC BACTERIA YERSINIA ENTEROCOLITICA OF SEROVARS O:3, O:5 OR O:6.30 AT APPLICATION OF PIEZOGRAVIMETRIC IMMUNOSENSOR

摘要

FIELD: immunology.;SUBSTANCE: through a flow microcell of 15-20 mcl volume one should pass the flow of phosphate buffer solution-carrier at the rate of about 30-90 mcl/min which should be supplemented with analyzed serumal sample at the volume of 200 mcl. The flow cell is supplied with a horizontally fixed piezogravimetric immunosensor at fluctuation frequency f_m and receptor covering based upon one of the LPC Yersinia enterocolitica of serovars O:3, O:5 or O:6.30. Then it is necessary to register the binding LPC Yersinia enterocolitica of serovars O:3, O:5 or O:6.30 with antibodies according to the decrease of sensor's fluctuation frequency from f_m up to f to detect the concentration of antibodies according to calibration graph being directly proportional against ▵f being equal to f_m-f. Application of the present innovation enables to decrease the procedure of analysis up to 10 min and, also, carry out a re-usable analysis after regeneration of sorbed LPC at sensitivity being 1.3 mcg/ml and detect concentration of antibodies ranged 10-100 mcg/ml.;EFFECT: higher accuracy and efficiency of detection.;8 ex, 1 tbl