METHOD FOR PREPARING INTERLEUKIN-8 FROM DONOR BLOOD NEUTROPHILES

摘要

FIELD: medicine, immunology, cytokines.;SUBSTANCE: method for preparing interleukin-8 (L-8) from donor blood neutrophiles involves isolating neutrophiles from peripheral blood in double gradient ficol-verografin, bringing about the neutrophile content in cell suspension to the concentration 5 x 10⁶ cells/ml, activation at temperature 37°C for 1 h followed by centrifugation and separation of supernatant containing the end product. For activation the cell suspension of neutrophiles in the concentration 5 x 10⁶/ml of Henx's solution is poured in plastic Petri dishes. Invention provides the development of a simple and available method providing the high level of interleukin-8 (IL-8) from neutrophiles of donor blood not requiring using expensive preparations.;EFFECT: improved preparing method.;1 tbl