SIMPLE, EFFECTIVE AND FAST METHOD FOR ENZYMATIC MODIFICATION AND SYNTHESIS OF NUCLEIC ACID IN VITRO USING MICROWAVE IRRADIATION

摘要

FIELD: biotechnology, in particular enzymatic reactions using nucleic acids as substrate.;SUBSTANCE: enzymatic treatment of nucleic acid is carried out with one or two enzymes in presence of microwave irradiation, buffer and other ingredients, being necessary for enzyme reaction.;EFFECT: simple, effective and fast method for enzymatic modification and synthesis of nucleic acid in vitro.;13 cl, 7 dwg, 1 tbl, 10 ex