METHOD FOR IN VITRO CRYOCONSERVATION OF MERISTEMS ISOLATED FROM STRAWBERRY (FRAGRARIA L) PLANTS

摘要

FIELD: biotechnology, agriculture, horticulture.;SUBSTANCE: meristem-donor plants are prepared for 2-8 weeks on solid, agar nutrient medium containing 4-10 % of sucrose. Precultivation of apexes isolated therefrom are carried out in presence of 0.79-0.81 M sucrose and 0.5-1.0 mg/l of paclobutrazole, namely [1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-pentane-3-ol], before drying and fast freezing.;EFFECT: effective method for strawberry cryoconservation.;2 dwg, 2 tbl, 2 ex