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RAPID GROUP METHOD FOR DIAGNOSING CATTLE HELMINTHOSES

机译:诊断牛蠕虫的快速分组方法

摘要

FIELD: veterinary medicine.;SUBSTANCE: method involves taking 10-100 samples of about 1 g large weight. They are thoroughly mixed with water and precipitated in single laboratory glass, filtering twice, washing the filtered suspension 3-5 times, centrifuging the precipitate 1-2 times at 1500 rpm, pouring the supernatant out and adding combined floatation fluid to the precipitate. Saturated ZnCl2 (2 kg per 1 l), NaCl (0.42 kg per 1 l) and sugar (1.67 kg per 1 l) solutions taken in 2:1:1 proportion are used as the floatation fluid. After having added floatation fluid, the floatation fluid precipitate is shaken up to produce suspension and centrifuged repeatedly in the same mode. The supernatant liquid is poured out and the floatation fluid is added to obtain convex meniscus. It is covered with object-plate for 3-5 min. Then, the liquid is studied with microscope on glass.;EFFECT: accelerated diagnosis method.;1 tbl
机译:领域:兽医学;物质:该方法涉及从10到100个重约1 g的大样本中取样。将它们与水充分混合并在单个实验室玻璃中沉淀,过滤两次,将过滤的悬浮液洗涤3-5次,将沉淀以1500 rpm离心1-2次,倒出上清液,然后将合并的浮选液添加到沉淀中。以2:1:1的比例使用饱和ZnCl 2 (2 kg每1 l),NaCl(0.42 kg每1 l)和糖(1.67 kg每1 l)溶液作为浮选液。加入浮选液后,将浮选液沉淀物摇动以产生悬浮液,并以相同模式重复离心。倒出上清液,并添加漂浮液以获得凸弯月面。用物板覆盖3-5分钟。然后,在玻璃上用显微镜研究液体。效果:加速诊断方法。1tbl

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