METHOD FOR PRODUCTION OF MESENCHYMAL STEM CELLS FROM MAMMALIAN MARROW AND MESENCHYMAL STEM CELL POPULATION PRODUCED BY THE SAME

摘要

FIELD: biotechnology, in particular cell therapy.;SUBSTANCE: invention relates to production of mesenchymal stem cells from human marrow. Uniform population of mesenchymal stem cells characterized with cell markers CD 44+, CD 45+, CD 34-, CD 90+, CD 105+, CD 106+ is isolated. Uniform cells are obtained on media interface during centrifugation of heparinized marrow and cells are subcultured in medium with addition cow foetus serum to produce cell biomass.;EFFECT: accelerated and inexpensive method for production of mesenchymal stem cells.;7 cl, 2 dwg, 2 tbl, 2 ex