A method for finding of an active substance which a destruction of tumor cells in the case of a tumor disease of a mammal or humans permits

摘要

A method for finding of an active substance which a destruction of tumor cells by initiating apoptosis by means of an effector in the case of a tumor disease of a mammal or humans, comprising the steps of:a1) providing a plurality of sowing of tumor cells by test series in each case in a culture vessel with cell culture medium, wherein the tumor cells or in addition, sown effector cells, which potentially apoptosis cellular effectors triggers the release, in each case in a cell density be seeded, which is suitable, in the case of the addition of a on the exterior of the tumor cells acting catalase inhibitor and incubation of the experimental batches under for the cultivation of the tumor cells or of the tumor cells and effector cells suitable conditions, to lead to apoptosis of the tumor cells,b1) adding at least in each case of a potential active ingredient to a respective one of the experimental batches, wherein at least one test batch remains, to which no potential active compound is added,c1) incubation of the experimental batches for the cultivation of the tumor cells under suitable conditions andd1) determining the test batch / of the experimental batches, in which / which the proportion of apoptotic tumor cells..