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A process for the spatially high-resolution investigation of samples
A process for the spatially high-resolution investigation of samples
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机译:对样本进行空间高分辨率研究的过程
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摘要
A process for the spatially high-resolution investigation of samples, preferably using a laser - grid - fluorescence microscope, wherein the sample to be examined (1) comprises a substance, the repeatedly from a first state (z1, a) in a second state (z2, b) is adapted to be transferred, wherein the first and second states (t1, a; z2, b) in at least one optical property differ from one another, comprising the steps of:a) by means of a switching signal (2), the substance to be detected in a test area (p) in the first state (z1, a) brought,b) by means of an optical signal (4), the second state (z2, b) is induced, whereby within the region of the (p) to be detected spatially limited partial regions are cut out in a targeted manner,c) by means of a test signal (7), the remaining first states (z1, a1, A2, A3) read out, andd) the steps a) to c) are repeated, wherein the optical signal (4) to the screening of the sample (1) in each repetition is displaced, is characterized in that the individual steps a) to d) in a sequence that is adapted to the respective measurement situation be carried out.
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