Method and medium for detecting / identifying microorganisms with esterase - and / or osidase - and / or peptidase - and / or sulfatase - and / or phosphatase activity

摘要

The invention concerns a medium for biological analysis by biochemical process comprising chromogenic and fluorogenic substrates reactive to enzymes specific (esterases and/or osidases and/or peptidases and/or sulphatases and/or phosphatases) of the searched strains. The invention aims at improving the sensitivity, initial translucency, stability and ease of use of such identification/detection media. This is achieved by the inventive medium which is in ready-to-use stable, liquid or gel form comprising at least an emulsifying stabilising agent such as ester of Sorbitan or of fatty acids/fatty acids and at least a solvent (dimethylsulphoxide). Said medium is obtained by mixing a stock solution of esterase and/or osidase and/or peptidase and/or sulphatase and/or phosphatase substrates, in the solvent and the emulsifying stabilising agent, with part of the constituents of the culture medium. The invention also concerns the preparation of such a medium and the use of ester of Sorbitan and of fatty acids/fatty acids as emulsifying stabiliser in a micro-organism detection/identification medium, in particular bacteria or yeasts, with esterase and/or osidase and/or peptidase and/or sulphatase and/or phosphatase activity.