Method and medium for the detecting/identifying microorganisms with esterase and/or r osidase and/or peptidase and/or sulfatase and/or phosphatase activity

摘要

The invention relates to a medium for biological analysis by biochemical means involving chromogenic or fluorogenic substrates that react with enzymes (esterases and/or osidases and/or peptidases and/or sulfatases and/or phosphatases) specific for the target strains. The object of the invention is to improve the sensitivity, initial translucence, stability and ease of use of such identification/detection media. This object is achieved by the medium according to the invention, which is in a stable, ready-to-use liquid or gel form and contains at least one emulsifying stabilizer of the fatty acid sorbitan ester/fatty acid type and at least one solvent (dimethyl sulfoxide). This medium is obtained by mixing a stock solution of esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase substrates in the solvent, and the emulsifying stabilizer, with some of the constituents of the culture medium. The invention further relates to the preparation of such a medium and to the use of a fatty acid sorbitan ester/fatty acids as an emulsifying stabilizer in a medium for the detection/identification of microorganisms, especially bacteria or yeasts, with esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities.