Tandem mass spectrometry (MS/MS) has been used extensively for solving various biological problems, such as structure identification of oligopeptides, nucleic acids, and nucleotides. One of the main advances of MS/MS is that it lends itself to the quantitative and qualitative analysis of certain analytes and complex mixtures with little or no clean up. Quantitative analysis of amino acids and acylcarnitines using tandem mass spectrometry used to screen neonatal dried blood spot samples for disorders in the metabolism of amino acids, organic acids and fatty acids is an emerging technology. The method described herein extends the use of tandem mass spectrometry to the analysis of α-hydroxyketones and hexose phosphates from blood spots for newborn screening. Thus, according to this invention one test can be used to screen a number of neonatal disorders and diseases.
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机译:串联质谱(MS / MS)已广泛用于解决各种生物学问题,例如寡肽,核酸和核苷酸的结构鉴定。 MS / MS的主要进步之一是,它可以对某些分析物和复杂混合物进行很少或没有净化的定量和定性分析。使用串联质谱法对氨基酸和酰基肉碱进行定量分析,用于筛选新生儿干血斑样品中的氨基酸,有机酸和脂肪酸代谢异常。本文所述的方法将串联质谱法的使用扩展到分析血点中的α-羟基酮和己糖磷酸酯以进行新生儿筛查。因此,根据本发明,一种测试可用于筛选许多新生儿疾病和疾病。
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