Designing oligonucleotides, useful in molecular biology process, comprises selecting data base of reference sequences and subset of sequences of desired organism, selecting and purifying selected oligonucleotides and obtaining their list

摘要

Designing oligonucleotides used in molecular biology process comprises selecting/creating a data base of reference sequences, i.e. an evaluating data base, selecting a subset of sequences of a desired organism to create a reference data base, i.e. a design data base, selecting oligonucleotides to be examined from the subset of sequences, purifying the selected oligonucleotides as per the criteria of hybridization specificity and thermodynamic stability and obtaining a list of oligonucleotides that satisfies all the above conditions to give oligonucleotides obtained by the method.