PRIMER SET FOR DETECTING DNA MARKER LINKED TO GENE LOCUS RELATING TO FAT-CONTENT IN CORN SEED AND ITS USE

摘要

PROBLEM TO BE SOLVED: To provide a primer set for detecting a DNA marker linked to a quantitative character gene locus relating to the fat content in a corn seed and to provide the use of the primer set.;SOLUTION: The invention provides a primer set for detecting a DNA marker linked to a QTL located on the first, fourth and sixth chromosomes participating in the fat content of a corn seed, a method for discriminating the genotype of the fat-content relating QTL by performing PCR with corn genome DNA using the primer set and discriminating by the difference of the length of the amplified DNA fragment, and a method for preparing a high fat-content lineage by selecting a body having a fat-content relating QTL derived from a high fat-content lineage and preparing a lineage having high fat content in the seed in the posterior lineages. The method enables easy and sure selection of individual body having increased fat content and enables the selection at a young seedling stage.;COPYRIGHT: (C)2008,JPO&INPIT