Specific detection method based on molecular identification of a variety of bacterial species of the genus Acinetobacter as well as nucleic acid fragments

摘要

The present invention, constituting a frame of rpoB gene and 77-100, and 35-56 and SEQ ID NO: 33 of the high mutation fragment of the rpoB gene and 11-32, and SEQ ID NO: 9 full-length rpoB gene, the rpoB gene fragment SEQ ID NO: 165-188 and 121-144 is a nucleic acid fragment of the non-coding sequences of a high variability to be, and further, a variety of Acinetobacter bacteria and oligonucleotide of species specific sequences derived from a fragment of the high mutation sequences A SEQ ID NO: 1 to 8 is the consensus oligonucleotide between species such species 24 the following: A. calcoacezicus (genospecies 1), A. baumannii (genospecies 2), 3, A genospecies. haemolylicus (genospecies 4), A. junii (genospecies 5), 6, A genetic species. johnsonii (genospecies 7), A. livoffli (genospecies 8), 9 genospecies, genospecies 10, 11, A genetic species. rndioresistens (genospecies 12), genetic species 13, 16, A genetic species. schindleri, A. ursingii, A. baylyi, A. bouvetii, A. genieri, A. grimontii, A. tandoii, A. tjernbergiae, A. towneri, A. related to those for parvus. Furthermore, in the method of detection by molecular identification of Acinetobacter bacterial species, the present invention relates to the use of the full-length gene rpoB said oligonucleotide, or the gene fragment,.