Operating the Hyphomycetes the DL- alanine content solution, it features that to resources it converts the L- alanine without excessive doing pyruvic acid. Simply be able to separate from the culture solution the Hyphomycetes, at the same time because pyruvic acid is not included in the culture solution, it is possible to produce the D- alanine efficiently. The D- alanine it is isolated from amount syneresis, if it should have processed with ion-exchange resin and the like. As these kind of Hyphomycetes, Aspergillus of marketing, penishiriumu being attached, it can use the mucor, rizopusu being attached, sashinera being attached, kuradosuporiumu being attached, torikoderuma being attached, yuroteiumu being attached, kaetomiumu being attached, oreobashideiumu being attached, geotorikamu being attached and paeshiromisesu being attached etc.
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