Malate synthase regulatory sequences for heterologous gene expression in Pichia

摘要

The present invention relates to an isolated DNA regulatory region of one gene malate synthase yeast Pichia pastoris from (Pichia pastoris) of (MLS1). The present invention relates to an expression of the heterologous protein can be expressed under the control of regulatory terminator region 'MLS1 3 and regulated promoters' MLS1 5 is introduced into Pichia pastoris (P.pastoris) host cell. Is suppressed in the medium containing glucose, MLS1 the promoter is derepressed in the case of acetic acid or glucose starvation conditions exist.