Methods and kits for determining the binding parameters of the biological affinity binding reaction

摘要

A by measuring the binding parameters, including adsorption rate constant and the dissociation constant of binding between the binding partner and biomolecules present in the liquid phase both in the process, including the binding step of the marker-free, the following steps, (a be coupled to either (a2) or biomolecule (a1) the binding partner on) solid surfaces, permitting the binding between the biomolecule and the binding partner in both the solid surface and (b) liquid phase, ( binding partner (c1) the biomolecule, or if has been immobilized on a solid surface to biological molecules according to (a2) step if if were immobilized on a solid surface to which the binding partners according to (a1) step c) If that, to allow it to couple the (c2) markers, that (d) a marker to produce a precipitate and by the determining a change of surface mass on the solid surface due to the formation of (e) precipitate wherein in that it comprises, a precipitate remains formed on the solid surface, and detecting the precipitate. [Selection] Figure Figure 1