Production method using the same and Corynebacterium ammoniagenes CJIP009 of microorganisms to produce a high yield of 5'-inosinic acid of 5'-inosinic acid

摘要

The present invention relates to a method of generating 5'-inosinic acid using the same microorganisms and the production of 5'-inosinic acid, more specifically the present invention, Corynebacterium ammoniagenes (Brevibacterium previous) (Corynebacterium ammoniagenes A mutant strain (ATCC-6872)), contrary to request adenine (Adenine), the microorganism of the present invention does not require guanine or xanthine (Xanthine) is (Guanine), but the growth is facilitated by adding this cell wall degradation is deemed to be the urease can be sucrose of urea is deficient, cell wall synthesis ability was partially deleted to be well secreted to the outside of the large amount of cells that are produced intracellularly and 5'-inosinic acid diversity and high glucose concentrations confers resistance to (Streptomycin) streptomycin antibiotics in order to respond effectively to contamination susceptibility (Lysozyme) lysozyme enzyme is high, occur during the fermentation process, are added in the course of culturing The carbon source such, or in the second half of the culture 5'-inosinic acid is accumulated in the culture in osmotic pressure of the external cell is increased, the normal physiological activity of the producing microorganism of 5'-inosinic acid inhibition For the purpose to strengthen the osmotic tolerance trait that can prevent that the bacteria growth slowed is, the generation of 5'-inosinic acid is reduced, and cells for high concentration solutes accumulated in cells outside To increase the intracellular concentration of proline concentration increases internally, an important role in osmoregulation (L- proline), and confers resistance to the analog to eliminate the influence of osmotic pressure more efficiently and also, to, in the invention relates to a microorganism that confers resistance to analogs of (glutamine) glutamine which essentially required in purine synthesis system, to accumulate directly in the culture medium high yield, a high concentration of 5'-inosinic acid there.