Spectral characteristic measuring apparatus and its method of measuring the fluorescent sample

摘要

PROBLEM TO BE SOLVED: To reduce the error due to the relative ultraviolet intensity change of an illuminating light to improve the measuring accuracy by correcting this change. ;SOLUTION: An arithmetic processing means 85 calculates the first and second total spectral emission brightness ratios Bt1 (λ), Bt2 (λ) when first and second illuminating means 3, 4 illuminate a fluorescent sample 1 from data obtained by spectroscopic means 5, 6, thereby obtaining a corrected total spectral emission brightness ratios Bt10 (λ). The weighting factor A (λ) is calculated, using a reference fluorescent sample 13, and stored in RAM 82. Using the weighting factor A (λ), a corrected total spectral emission brightness ratios Bt10 (λ) and the second total spectral emission brightness ratio Bt2 (λ) are linearly combined to calculate the total spectral emission brightness ratios Bt (λ) of the sample 1.;COPYRIGHT: (C)2000,JPO