首页>
外国专利>
Fed-batch fermentation method and medium for producing plasmid DNA in Escherichia coli on a manufacturing scale
Fed-batch fermentation method and medium for producing plasmid DNA in Escherichia coli on a manufacturing scale
展开▼
机译:分批分批发酵的方法和在大肠杆菌中生产质粒DNA的培养基
展开▼
页面导航
摘要
著录项
相似文献
摘要
A process for producing plasmid DNA E. coli cells comprises a pre-culture and fed-batch process. The culture media of the batch phase and the culture medium added during the feeding phase are chemically defined. The culture medium of the feeding phase contains a growth-limiting substrate and is added at a feeding rate that follows a pre-defined exponential function, thereby controlling the specific growth rate at a pre-defined value. The process results in high yield and homogeneity of plasmid DNA.
展开▼