Site-specific protein built of heavy atom-containing non-natural amino acids for the crystal structure determination

摘要

I to provide a composition and other translation system and an orthogonal aminoacyl-tRNA synthetases that load preferentially brominated or iodinated amino acid to the orthogonal tRNA. Also described is a method and a kit for producing a nucleic acid encoding the synthetase, a protein incorporating (iodinated amino acids or brominated, for example) heavy atom-containing amino acid. Described a method of determining the structure (protein heavy atom is specifically incorporated into proteins, for example) protein by using an orthogonal tRNA / aminoacyl-tRNA synthetase pair.