Recombinant bovine pancreatic DNase I having a high specific activity

摘要

PROBLEM TO BE SOLVED: To provide a substitutional supply source for bovine pancreatic deoxyribonulcease I, having a high cost effect.;SOLUTION: A method for producing a bovine pancreatic protein includes (a) a process for providing a vector containing a nucleotide sequence which encodes a preprotein comprising the bovine pancreatic protein and a signal peptide, (b) another process for transforming a methylotrophic yeast strain by the vector, (c) a third process for culturing the transformed methylotrophic yeast strain in a growth medium containing nutrients and methanol, wherein the methylotrophic yeast strain expresses the bovine pancreatic protein and secretes the protein in the growth medium, and (d) the other process for purifying the bovine pancreatic protein from the growth medium, so that the bovine pancreatic protein purified in the process (d) has a deoxyribonulcease specific activity of at least 6,000 units per mg of the purified protein.;COPYRIGHT: (C)2004,JPO&NCIPI