The arrangement which makes the revelation of the gene promote with minor low temperature

摘要

It rearranges making use of the mammal cell and it designates that it increases the protein which is produced in DNA method as purpose. Nucleotide arrangement: x₁ ccccx₆ x₇ x₈(In formula, x₁, displays t, g or a, x₆, g and a, or displays c, x₇, c, displays a or t, x₈, c, displays a or t)Or when it uses the minor low temperature transcriptional control element which one includes complimentary nucleotide arrangement in this arrangement at least cultures in minor low temperature as enhansa, copying is promoted. In addition 5 ' on side of cord/code arrangement when it arranges the minor low temperature translational control element of arrangement number 6 3 ' on side of the promoter, cultures in minor low temperature translation is promoted.