Modified human hepatitis C virus genomic RNA with autonomous replication ability

摘要

The present invention relates to 5 'untranslated region, core protein coding sequence, E1 protein coding sequence, p7 protein coding sequence, E2 protein coding sequence, NS2 protein coding sequence, NS3 protein of two or more types of hepatitis C virus genomic RNA. A modified hepatitis C virus comprising a coding sequence, an NS4A protein coding sequence, an NS4B protein coding sequence, an NS5A protein coding sequence, an NS5B protein coding sequence, and a base sequence comprising a 3 ′ untranslated region and having autonomous replication ability Provide genomic RNA. In particular, the RNA sequence portion encoding NS3 to NS4, NS5A, NS5B protein of hepatitis C virus genomic RNA is changed to the RNA partial sequence encoding NS3, NS4, NS5A and NS5B proteins of JFH1 strain represented by SEQ ID NO: 1. The present invention relates to a modified hepatitis C virus genomic RNA that has acquired autonomous replication ability by substitution.